During the year 2005, the lab continued its research progress along the same lines that we had previously.
     1) Genome annotation
     2) Understanding protein function in terms of networks
     3) Structural genomics
     4) Biophysical analysis of macromolecular motions.

Additionally, we also wrote a number of papers that explored various social aspects of large-scale scientific collaboration and we engaged in a number of multi-disciplinary collaborations.

In relation to the genomics, we accomplished two things.  First of all, we had two papers, Harrison et al and Zheng et al, which looked at transcribed pseudogenes in the human genome and tried to show that even though pseudogenes are thought of as dead copies of genes, they might actually have some activity in them in relationship to transcription.  In Royce et al, 2005, we developed some approaches for scoring microarrays, which is very important for the analysis of these intergenic regions.  In Balasubramanian et al, we also continued on our genomic analysis, focusing on variations merging nets with a particularly well-known protein family and trying to predict which SNPs were deleterious.

Continuing the structural work, Alexandrov et al and Voss et al, reported work using normal modes to predict RNA packing and to look at motions.

In protein networks, Lu et al assessed the degree to which we could predict networks, and in Douglas et al, we developed a new tool for visualizing networks derived from connected genes, but also networks related to scientific co-publication.

In the broader context of connecting computing to molecular biology, we wrote a number of papers on the ideas behind database interoperation.  In Cheung et al, we developed a prototype system for assessing the interoperation of databases related to yeast.  In Smith et al, we looked at the degree to which computer security consideration significantly impede database interoperation.

Finally, we also had a number of novel experimental collaborations this year. At the end of the year, in Ptacek et al, we were participants in a large multi-disciplinary team that published a paper unraveling the phosphorylome, the network of all protein-protein interactions involved in phosphorylation, an important signalling process.

Design optimization methods for genomic DNA tiling arrays.
P Bertone, V Trifonov, JS Rozowsky, F Schubert, O Emanuelsson, J Karro, MY Kao, M Snyder, M Gerstein (2006). Genome Res 16: 271-81.

Inferring Protein-Protein Interactions Using Interaction Network Topologies
A Paccanaro, V Trifonov, H Yu, M Gerstein (2005). International Joint Conference on Neural Networks (IJCNN, Jul. 31-Aug. 4, Montreal, Canada), pages 161 - 166, vol. 1

Protein Interaction Prediction by Integrating Genomic Features and Protein Interaction Network Analysis
LJ Lu, Y Xia, H Yu, A Rives, H Lu, F Schubert, M Gerstein (2005). Data Analysis and Visualization in Genomics and Proteomics (Wiley, NY)

Biochemical and genetic analysis of the yeast proteome with a movable ORF collection.
DM Gelperin, MA White, ML Wilkinson, Y Kon, LA Kung, KJ Wise, N Lopez-Hoyo, L Jiang, S Piccirillo, H Yu, M Gerstein, ME Dumont, EM Phizicky, M Snyder, EJ Grayhack (2005). Genes Dev 19: 2816-26.

Global analysis of protein phosphorylation in yeast.
J Ptacek, G Devgan, G Michaud, H Zhu, X Zhu, J Fasolo, H Guo, G Jona, A Breitkreutz, R Sopko, RR McCartney, MC Schmidt, N Rachidi, SJ Lee, AS Mah, L Meng, MJ Stark, DF Stern, C De Virgilio, M Tyers, B Andrews, M Gerstein, B Schweitzer, PF Predki, M Snyder (2005). Nature 438: 679-84.

Global changes in STAT target selection and transcription regulation upon interferon treatments.
SE Hartman, P Bertone, AK Nath, TE Royce, M Gerstein, S Weissman, M Snyder (2005). Genes Dev 19: 2953-68.

Network security and data integrity in academia: an assessment and a proposal for large-scale archiving.
A Smith, D Greenbaum, SM Douglas, M Long, M Gerstein (2005). Genome Biol 6: 119.

PubNet: a flexible system for visualizing literature derived networks.
SM Douglas, GT Montelione, M Gerstein (2005). Genome Biol 6: R80.

Proton sensitivity of ASIC1 appeared with the rise of fishes by changes of residues in the region that follows TM1 in the ectodomain of the channel.
T Coric, D Zheng, M Gerstein, CM Canessa (2005). J Physiol 568: 725-35.

Assessing the limits of genomic data integration for predicting protein networks.
LJ Lu, Y Xia, A Paccanaro, H Yu, M Gerstein (2005). Genome Res 15: 945-53.

Issues in the analysis of oligonucleotide tiling microarrays for transcript mapping.
TE Royce, JS Rozowsky, P Bertone, M Samanta, V Stolc, S Weissman, M Snyder, M Gerstein (2005). Trends Genet 21: 466-75.

YeastHub: a semantic web use case for integrating data in the life sciences domain.
KH Cheung, KY Yip, A Smith, R Deknikker, A Masiar, M Gerstein (2005). Bioinformatics 21 Suppl 1: i85-96.

Integrated pseudogene annotation for human chromosome 22: evidence for transcription.
D Zheng, Z Zhang, PM Harrison, J Karro, N Carriero, M Gerstein (2005). J Mol Biol 349: 27-45.

Applications of DNA tiling arrays to experimental genome annotation and regulatory pathway discovery.
P Bertone, M Gerstein, M Snyder (2005). Chromosome Res 13: 259-74.

Multi-species microarrays reveal the effect of sequence divergence on gene expression profiles.
Y Gilad, SA Rifkin, P Bertone, M Gerstein, KP White (2005). Genome Res 15: 674-80.

Transcribed processed pseudogenes in the human genome: an intermediate form of expressed retrosequence lacking protein-coding ability.
PM Harrison, D Zheng, Z Zhang, N Carriero, M Gerstein (2005). Nucleic Acids Res 33: 2374-83.

Use of thioredoxin as a reporter to identify a subset of Escherichia coli signal sequences that promote signal recognition particle-dependent translocation.
D Huber, D Boyd, Y Xia, MH Olma, M Gerstein, J Beckwith (2005). J Bacteriol 187: 2983-91.

Robotic cloning and Protein Production Platform of the Northeast Structural Genomics Consortium.
TB Acton, KC Gunsalus, R Xiao, LC Ma, J Aramini, MC Baran, YW Chiang, T Climent, B Cooper, NG Denissova, SM Douglas, JK Everett, CK Ho, D Macapagal, PK Rajan, R Shastry, LY Shih, GV Swapna, M Wilson, M Wu, M Gerstein, M Inouye, JF Hunt, GT Montelione (2005). Methods Enzymol 394: 210-43.

Sequence variation in G-protein-coupled receptors: analysis of single nucleotide polymorphisms.
S Balasubramanian, Y Xia, E Freinkman, M Gerstein (2005). Nucleic Acids Res 33: 1710-21.

The temporal patterning microRNA let-7 regulates several transcription factors at the larval to adult transition in C. elegans.
H Grosshans, T Johnson, KL Reinert, M Gerstein, FJ Slack (2005). Dev Cell 8: 321-30.

Normal modes for predicting protein motions: a comprehensive database assessment and associated Web tool.
V Alexandrov, U Lehnert, N Echols, D Milburn, D Engelman, M Gerstein (2005). Protein Sci 14: 633-43.

Calculation of standard atomic volumes for RNA and comparison with proteins: RNA is packed more tightly.
NR Voss, M Gerstein (2005). J Mol Biol 346: 477-92.

A high productivity/low maintenance approach to high-performance computation for biomedicine: four case studies.
N Carriero, MV Osier, KH Cheung, PL Miller, M Gerstein, H Zhao, B Wu, S Rifkin, J Chang, H Zhang, K White, K Williams, M Schultz (2005). J Am Med Inform Assoc 12: 90-8.

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